草業(yè)科學專用的葉綠素熒光成像系統(tǒng)Weed-PAM
日期:2018-05-09 14:53:00

草業(yè)科學-葉綠素熒光成像系統(tǒng)Weed-PAM

基于功能強大的IMAG-PAM,改進升級后適用于野外雜草,草坪等光合作用研究

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主要功能

l 成像功能:對Ft、FoFm、Fv/Fm、F、Fm’、Y(II)、Y(NO)、Y(NPQ)NPQ、qNqP、qLETR、Abs.NIR、Red等至少17種參數(shù)進行成像分析。測定調(diào)節(jié)性能量耗散Y(NPQ),反映植物光保護能力,測定非調(diào)節(jié)性能量耗散Y(NO),反映植物光損傷程度。

l 程序測量功能:可程序測量熒光誘導曲線、快速光曲線和暗弛豫,也可手動測量;在測量過程中能自動分析所有熒光參數(shù)的變化趨勢

l AOI功能:可在測量前或測量后任意選擇感興趣的區(qū)域(AOI),程序?qū)⒆詣訉x擇的AOI的數(shù)據(jù)進行變化趨勢分析,并在報告文件中顯示相關(guān)AOI的數(shù)據(jù)。所有報告文件中顯示的數(shù)據(jù)都可導出到EXCEL文件中。

l 成像異質(zhì)性分析功能:對任意參數(shù)任意時間的成像,可在圖像上任意選取兩點,軟件自動對兩點間的數(shù)據(jù)進行橫向異質(zhì)性分析,并可導出到EXCEL文件中。

l 成像數(shù)據(jù)范圍分析功能:對任意參數(shù)任意時間的成像,可分析任意兩個熒光數(shù)值之間有多少個像素點,多少面積(cm2)。

l 突變株篩選功能:可跟據(jù)成像結(jié)果快速篩選光合、產(chǎn)氫/油、抗逆(抗鹽、抗旱、抗病等)等突變株。

l 吸光系數(shù)測量功能:快速測量葉片的吸光系數(shù)。吸光系數(shù)測量光源: 16個紅光(650 nm)和16個近紅外(780 nmLED,用于測量植物葉片或藻類樣品PAR吸光系數(shù)。

成像參數(shù)

Fo, Fm, F, Ft, Fm', Fv/Fm, Y(II), qL, qP, qN, NPQ, Y(NPQ), Y(NO), ETR, Abs, NIR和Red等

技術(shù)參數(shù)

Maxi探頭(藍光版)藍色LED,450 nm


Maxi探頭(紅光版)紅色LED650 nm


Mini探頭(藍光版藍色LED,460 nm

 

Mini探頭(紅光版紅色LED,620 nm


應(yīng)用領(lǐng)域

光合作用研究:可以在相同的條件下同時對大量樣品進行成像

l 植物病理學:病斑部位(包括肉眼不可見時)成像以及病斑擴散的時空動力學

l 植物脅迫生 理學:肉眼不可見脅迫損傷的早期檢測

l 遺傳育種:出苗后大規(guī)模快速篩選高光合/抗旱/抗熱/抗凍/抗病等植株

l 突變株篩選:快速篩選模式植物的光合突變株、抗逆突變株、產(chǎn)氫微藻突變株等

l 分子生物學:宏觀水平上檢測樣品的綠色熒光蛋白(GFP)熒光

l 其它多種擴展研究



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參考文獻

數(shù)據(jù)來源:光合作用文獻 Endnote 數(shù)據(jù)庫,更新至 2021 年 1 月,文獻數(shù)量超過 10000 篇

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